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Planta Med 2014; 80(02/03): 243-248
DOI: 10.1055/s-0033-1360301
Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Fast Quantification of S-adenosyl-L-methionine in Dietary Health Products Utilizing Reversed-Phase High-performance Liquid Chromatography: Teaching an Old Method New Tricks

Jing-Ru Liou   *
1   Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
,
Li-Yeh Chuang   *
2   Department of Chemical Engineering, Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung, Taiwan
,
Yuan-Han Yang
3   Department of Neurology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan
4   Department of Masterʼs Program in Neurology, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
,
Tung-Ying Wu
1   Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
,
Mohamed El-Shazly
5   Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity, Abassia, Cairo, Egypt
,
Shyh-Jong Wu
6   Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan
,
Tusty-Jiuan Hsieh
7   Department of Genome Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
,
Yang-Chang Wu
8   School of Pharmacy, College of Pharmacy, China Medical University, Taichung, Taiwan
9   Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung, Taiwan
10   Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan
,
Jiumn-Yih Wu
2   Department of Chemical Engineering, Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung, Taiwan
,
Fang-Rong Chang
1   Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
11   Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
12   Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung, Taiwan
› Author Affiliations
Further Information

Publication History

received 25 August 2013
revised 14 November 2013

accepted 29 December 2013

Publication Date:
31 January 2014 (online)

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Abstract

S-adenosyl-L-methionine is a ubiquitous methyl donor in living bodies. It is known to participate in several physiological processes including homocysteine metabolism and glutathione synthesis regulation, and cellular antioxidant mechanism. S-adenosyl-L-methionine containing dietary supplements has been prescribed recently for the treatment of depression, arthritis, and liver diseases with encouraging results. The development of an efficient analytical protocol for S-adenosyl-L-methionine containing dietary supplements is crucial for maintaining product quality and consumer health. In this study, the S-adenosyl-L-methionine content of several yeast products and commercial healthy food product samples was quantitatively analyzed utilizing HPLC. The chromatographic separation was achieved on a reversed-phase column and 2 % acetonitrile with a 98 % ammonium-acetate mobile phase under pH 4.5, with a flow rate of 1.0 mL/min. The wavelength used for detection with the UV detector was 254 nm. The total analysis time was short and the target compound showed a well-defined peak. The correlation coefficient of the regression curve showed good linearity and sensitivity with r = 0.999. All experiments were replicated five times and the relative standard deviations as well as the relative error values were all less than 3 %. Moreover, the achieved precision and accuracy values were high with 97.4–100.9 % recovery. Qualitative determination of S-adenosyl-L-methionine in the tested products was achieved using NMR and LC-MS techniques. The developed protocol is robust, fast, and suitable for the quality control analysis of yeast and commercial S-adenosyl-L-methionine products.

Key words

HPLC - S-adenosyl-L-methionine - Saccharomyces cerevisiae (yeast) - LC-MS

* These authors contributed equally to this work.


Supporting Information